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Figure 1 | BMC Biochemistry

Figure 1

From: Nuclear annexin II negatively regulates growth of LNCaP cells and substitution of ser 11 and 25 to glu prevents nucleo-cytoplasmic shuttling of annexin II

Figure 1

Annexin II is phosphorylated in nuclear extract of K562 cells. Human K562 cells were fractionated into nuclear extract (NE) and cytosolic extract (CE). One aliquot of NE and CE was treated with CIAP prior to SDS-PAGE. Untreated CE and NE were subjected to incubation in the phosphatase buffer without CIAP. 20 μg protein from each extract were subjected to SDS-PAGE and immunoblotting with anti-annexin II antisera. Positions of the phosphorylated (upper two) and dephosphorylated annexin II are indicated by arrows.

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BMC Biochemistry

ISSN: 1471-2091

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