Figure 2

Identification of reaction products of CoA hydrolysis. Reaction mixtures containing 0.5 mM CoA were incubated at 37°C for 20 min with or without 0.1 μg Trx-Y87G2A.14 fusion protein and the products separated by HPLC as described in Materials and methods. Without enzyme (------), with enzyme (______), gradient (— — — — —). Positions of authentic standards are indicated.