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Table 1 Oligonucleotides used in preparation of L1 mutants

From: Probing substrate binding to Metallo-β-Lactamase L1 from Stenotrophomonas maltophilia by using site-directed mutagenesis

Primer Sequence
pUCMSZFor CTATgCggCATCAgAgCAgATT
M13Rev gATAACAATTTCACACAggA
Y228AFor CTgAgTgCACCgggCgCCCAgCTgCAgggAAAC
Y228ARev gTTTCCCTgCAgCTgggCgCCCggTgCACTCAg
Y228FFor CTgAgTgCACCgggCTTCCAgCTgCAgggAAAC
Y228FRev gTTTCCCTgCAgCTggAAgCCCggTgCACTCAg
S224DFor TACgCCgACAgCCTggACgCACCgggCTACCAg
S224DRev CAggTAgCCCggTgCgTCCAggCTgTCggCgTA
S224AFor TACgCCgACAgCCTggCCgCACCgggCTACCAg
S224ARev CAggTAgCCCggTgCggCCAggCTgTCggCgTA
S224KFor TACgCCgACAgCCTgAAggCACCgggCTACCAg
S224KRev CAggTAgCCCggTgCCTCCAggCTgTCggCgTA
F158AFor AgCgATgACCTgCACgCCggCgATggCATCACC
F158ARev ggTgATgCCATCgCCggCgTgCAggTCATCgCT
I164AFor CACTTCggCgATggCgCCACCTACCCgCCTgCC
I164ARev ggCAggCgggTAggTggCgCCATCgCCgAAgTg
N233LFor TACCAgCTgCAgggACTgCCCCgTTATCCgCAC
N233LRev gTgCggATAACggggCAgTCCCTgCAgCTggTA
N233DFor TACCAgCTgCAgggAgACCCCCgTTATCCgCAC
N233DRev gTgCggATAACgggggTCTCCCTgCAgCTggTA