Table 1 Oligonucleotides used in preparation of L1 mutants
Primer | Sequence |
|---|---|
pUCMSZFor | CTATgCggCATCAgAgCAgATT |
M13Rev | gATAACAATTTCACACAggA |
Y228AFor | CTgAgTgCACCgggCgCCCAgCTgCAgggAAAC |
Y228ARev | gTTTCCCTgCAgCTgggCgCCCggTgCACTCAg |
Y228FFor | CTgAgTgCACCgggCTTCCAgCTgCAgggAAAC |
Y228FRev | gTTTCCCTgCAgCTggAAgCCCggTgCACTCAg |
S224DFor | TACgCCgACAgCCTggACgCACCgggCTACCAg |
S224DRev | CAggTAgCCCggTgCgTCCAggCTgTCggCgTA |
S224AFor | TACgCCgACAgCCTggCCgCACCgggCTACCAg |
S224ARev | CAggTAgCCCggTgCggCCAggCTgTCggCgTA |
S224KFor | TACgCCgACAgCCTgAAggCACCgggCTACCAg |
S224KRev | CAggTAgCCCggTgCCTCCAggCTgTCggCgTA |
F158AFor | AgCgATgACCTgCACgCCggCgATggCATCACC |
F158ARev | ggTgATgCCATCgCCggCgTgCAggTCATCgCT |
I164AFor | CACTTCggCgATggCgCCACCTACCCgCCTgCC |
I164ARev | ggCAggCgggTAggTggCgCCATCgCCgAAgTg |
N233LFor | TACCAgCTgCAgggACTgCCCCgTTATCCgCAC |
N233LRev | gTgCggATAACggggCAgTCCCTgCAgCTggTA |
N233DFor | TACCAgCTgCAgggAgACCCCCgTTATCCgCAC |
N233DRev | gTgCggATAACgggggTCTCCCTgCAgCTggTA |