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Table 6 Km and KS values for Wild-type L1 and mutants with nitrocefin as substrate

From: Probing substrate binding to Metallo-β-Lactamase L1 from Stenotrophomonas maltophilia by using site-directed mutagenesis

Enzyme Km (μM) KS (μM)
Wild-type 4 ± 1 38 ± 5
S224A 7 ± 1 39 ± 10
S224D 11 ± 5 213 ± 63
S224K 14 ± 2 33 ± 5
F158A 4 ± 1 N.D.
I164A 8 ± 2 31 ± 11
Y228A 23 ± 3 85 ± 9
Y228F 36 ± 16 22 ± 6
N233D 9 ± 2 26 ± 8
N233L 7 ± 2 25 ± 7
  1. N.D. – not determined