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Table 6 Km and KS values for Wild-type L1 and mutants with nitrocefin as substrate

From: Probing substrate binding to Metallo-β-Lactamase L1 from Stenotrophomonas maltophilia by using site-directed mutagenesis

Enzyme

Km (μM)

KS (μM)

Wild-type

4 ± 1

38 ± 5

S224A

7 ± 1

39 ± 10

S224D

11 ± 5

213 ± 63

S224K

14 ± 2

33 ± 5

F158A

4 ± 1

N.D.

I164A

8 ± 2

31 ± 11

Y228A

23 ± 3

85 ± 9

Y228F

36 ± 16

22 ± 6

N233D

9 ± 2

26 ± 8

N233L

7 ± 2

25 ± 7

  1. N.D. – not determined
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BMC Biochemistry

ISSN: 1471-2091

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