Table 5 Steady-state kinetic constants for hydrolysis of carbapenems by wild-type L1 and L1 mutants

w.t.

32 ± 1

134 ± 4

0.42

57 ± 7

370 ± 5

0.65

15 ± 4

157 ± 9

1.0

S224A

34 ± 5

56 ± 2

0.16

29 ± 4

100 ± 3

0.34

50 ± 10

244 ± 1

0.49

S224K

100 ± 22

43 ± 2

0.043

60 ± 4

14 ± 1

0.023

12 ± 1

212 ± 1

1.8

S224D

64 ± 7

22 ± 1

0.034

42 ± 4

17 ± 1

0.040

132 ± 23

69 ± 7

0.052

I164A

55 ± 3

112 ± 1

0.20

92 ± 11

570 ± 43

0.62

14 ± 1

96 ± 3

0.69

F158A

50 ± 11

70 ± 5

0.14

100 ± 20

370 ± 50

0.37

7 ± 2

36 ± 2

0.51

Y228A

175 ± 25

21 ± 2

0.012

350 ± 94

134 ± 36

0.038

4.5 ± 0.2

26 ± 1

0.58

Y228F

150 ± 23

51 ± 4

0.034

107 ± 13

83 ± 10

0.078

13 ± 1

70 ± 1

0.54

N233L

29 ± 3

105 ± 6

0.36

36 ± 5

250 ± 20

0.69

12 ± 1

67 ± 3

0.56

N233D

28 ± 8

7 ± 1

0.025

71 ± 16

158 ± 13

0.22

16 ± 4

3.5 ± 0.1

0.022