Table 4 Steady-state kinetic constants for hydrolysis of penicillins by wild-type L1 and L1 mutants.

w.t.

38 ± 12

600 ± 100

1.6

55 ± 5

520 ± 10

0.95

S224A

70 ± 20

580 ± 100

0.83

125 ± 13

339 ± 1

0.27

S224K

44 ± 8

124 ± 12

0.28

25 ± 3

152 ± 2

0.61

S224D

1600 ± 200

42 ± 9

0.0026

1100 ± 240

10 ± 1

0.00091

I164A

60 ± 5

698 ± 100

1.2

43 ± 3

524 ± 100

1.2

F158A

50 ± 5

138 ± 10

0.28

165 ± 20

270 ± 30

0.16

Y228A

410 ± 60

609 ± 64

0.15

710 ± 74

443 ± 10

0.062

Y228F

140 ± 14

630 ± 30

0.45

271 ± 40

243 ± 30

0.090

N233L

33 ± 9

184 ± 35

0.56

90 ± 20

508 ± 40

0.56

N233D

60 ± 2

440 ± 86

0.73

117 ± 18

621 ± 31

0.53