nitrocefin | cefoxitin | cefaclor | cephalothin | |||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
Enzyme | Km (μM) | kcat (s-1) | kcat/Km × 107 M-1 s-1 | Km (μM) | kcat (s-1) | kcat/Km × 107 M-1 s-1 | Km (μM) | kcat (s-1) | kcat/Km × 107 M-1 s-1 | Km (μM) | kcat (s-1) | kcat/Km × 10-7 M-1 s-1 |
w.t. | 4 ± 1 | 41 ± 1 | 1.0 | 1.1 ± 0.1 | 1.9 ± 0.1 | 0.17 | 13 ± 1 | 42 ± 1 | 0.32 | 8.9 ± 1.5 | 82 ± 5 | 0.92 |
S224A | 7 ± 1 | 48 ± 5 | 0.69 | 3.0 + 0.5 | 1.0 ± 0.1 | 0.033 | 14 ± 2 | 14 ± 1 | 0.10 | 3.6 ± 0.1 | 19.8 ± 0.2 | 0.55 |
S224K | 14 ± 2 | 48 ± 10 | 0.34 | 2.0 ± 0.3 | 0.60 ± 0.06 | 0.030 | 13 ± 2 | 6.5 ± 0.3 | 0.050 | 6.2 ± 0.6 | 26 ± 1 | 0.42 |
S224D | 11 ± 5 | 2.3 ± 0.4 | 0.021 | 50 ± 6 | 1.4 ± 0.2 | 0.0028 | 215 ± 17 | 3 ± 1 | 0.0014 | 75 ± 7 | 32 ± 2 | 0.043 |
I164A | 8 ± 2 | 130 ± 30 | 1.6 | 11 ± 1 | 8 ± 1 | 0.073 | 3.3 + 0.5 | 30 ± 3 | 0.91 | 16 ± 1 | 146 ± 2 | 0.91 |
F158A | 123 ± 20 | 1290 ± 20 | 1.0 | 11 ± 2 | 16 ± 2 | 0.15 | 135 ± 23 | 99 ± 9 | 0.073 | 63 ± 17 | 353 ± 35 | 0.56 |
Y228A | 23 ± 3 | 72 ± 2 | 0.31 | 10 ± 2 | 5.8 ± 0.3 | 0.058 | 550 ± 100 | 40 ± 3 | 0.0073 | 290 ± 60 | 320 ± 40 | 0.11 |
Y228F | 36 ± 16 | 81 ± 18 | 0.23 | 8.2 ± 1.1 | 5.5 ± 0.5 | 0.067 | 240 + 60 | 74 ± 4 | 0.031 | 58 ± 7 | 190 ± 50 | 0.33 |
N233L | 7 ± 2 | 62 ± 12 | 0.89 | 4.4 ± 1.6 | 0.90 ± 0.17 | 0.020 | 14± 2 | 32 ± 1 | 0.23 | 8.0 + 0.7 | 51 ± 1 | 0.64 |
N233D | 9 ± 2 | 21 ± 2 | 0.23 | 1.1 ± 0.2 | 1.1 ± 0.1 | 0.10 | 25 ± 5 | 34 ± 3 | 0.14 | 18 ± 4 | 65 ± 2 | 0.36 |