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Figure 2 | BMC Biochemistry

Figure 2

From: Probing substrate binding to Metallo-β-Lactamase L1 from Stenotrophomonas maltophilia by using site-directed mutagenesis

Figure 2

Intermediate formation by wild-type L1 and L1 mutants. The spectra were collected using rapid scanning Vis studies, and the absorbance values at 668 nm were converted to concentration values as described in Materials and Methods. Typical reactions were conducted with 25 μM L1 (or mutant) and 5 μM nitrocefin in 50 mM cacodylate, pH 7.0, containing 100 μM ZnCl2 at 25°C.

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