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Figure 4 | BMC Biochemistry

Figure 4

From: Src Kinase becomes preferentially associated with the VEGFR, KDR/Flk-1, following VEGF stimulation of vascular endothelial cells

Figure 4

Coimmunoprecipitation of Fyn with Flt-1, and Yes with Flt-1.HUVECs were serum-starved (-), or starved and then treated with VEGF (15 ng/mL for 15 min) (+). The cell extracts were then subjected to immunoprecipitation-Western blotting experiments. (A) Cell extracts from the indicated treatment conditions were immunoprecipitated using α-Fyn, and immunoblotted using α-Flt-1 (H-225), α-KDR/Flk-1 (A-3), or α-Fyn. (B) Cell extracts from the indicated treatment conditions were immunoprecipitated using α-Yes, and immunoblotted using α-Flt-1 (H-225), α-KDR/Flk-1 (A-3), or α-Yes. (C) Cell extracts from the indicated treatment conditions were immunoprecipitated using α-Flt-1 (H-225), and immunoblotted using α-phosphotyrosine (4G10) or α-Flt-1 (H-225). (D) Cell extracts from the indicated treatment conditions were immunoprecipitated using α-KDR/Flk-1 (N-931), and immunoblotted using α-phosphotyrosine (4G10) or α-KDR/Flk-1 (A-3).

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