Skip to main content

Table 4 Hydrodynamic analysis of T701-Myo1c.

From: Calmodulin binding to recombinant myosin-1c and myosin-1c IQ peptides

Property

Temperature

T701-Myo1c

100 μM EGTA

T701-Myo1c

25 μM CaCl2

Sedimentation coefficient

4°C

25°C

4.64 ± 0.11 S (n = 4)

4.27 ± 0.21 S (n = 3)

  

3.83 ± 0.06 S (n = 11)

3.13 ± 0.30 S (n = 7)

Stokes radius

4°C

25°C

4.52 ± 0.06 nm (n = 4)

4.19 ± 0.11 nm (n = 6)

  

4.74 ± 0.13 nm (n = 6)

3.78 ± 0.07 nm (n = 6)

Molecular mass

4°C

25°C

87 ± 5 kD

74 ± 6 kD

  

75 ± 3 kD

49 ± 5 kD

Frictional ratio

4°C

25°C

1.5 ± 0.1

1.5 ± 0.1

  

1.7 ± 0.1

1.6 ± 0.2

Calmodulins per molecule

4°C

25°C

2.5 ± 0.1

1.8 ± 0.2

  

1.8 ± 0.2

0.3 ± 0.3

  1. Analysis was carried out as described for Table 3 using purified T701-Myo1c.