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Table 2 Interaction of Myo1c IQ peptides with unlabeled calmodulin

From: Calmodulin binding to recombinant myosin-1c and myosin-1c IQ peptides

Peptide

Apparent Kd,

EGTA

Apparent Kd,

CaCl2

IQ1

4.0 ± 0.8 μM

56 ± 13 μM

IQ2

4.2 ± 2.0 μM

70 ± 15 μM

IQ3

1.7 ± 0.7 μM

3.2 ± 1.1 μM

  1. Quenching of Alexa-calmodulin fluorescence was used to estimate binding affinity of IQ peptides to calmodulin using equation (7). Values reported are means ± standard error (n = 3 to 4 independent experiments for each value). IQ4 affinities were not accurately measured under conditions of this assay, but were >100 μM.