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Figure 3 | BMC Biochemistry

Figure 3

From: Calmodulin binding to recombinant myosin-1c and myosin-1c IQ peptides

Figure 3

Alexa-calmodulin binding to Myo1c IQ peptides in solution Binding of IQ peptides to Alexa-calmodulin (100 nM) at room temperature with 150 mM KCl and 100 μM EGTA. A, IQ peptides quench Alexa-calmodulin fluorescence; unlabeled calmodulin reverses the quench. IQ peptides (25 μM) and unlabeled calmodulin (75 μM) were added as indicated. Control, Alexa-calmodulin alone; +IQ, Alexa-calmodulin plus IQ peptide; +IQ, +CaM, IQ peptide plus unlabeled calmodulin; IQ only, IQ peptides with no Alexa-calmodulin. B, Alexa-calmodulin (100 nM) was incubated with increasing concentrations of soluble IQ peptides. Total IQ peptide concentration added is plotted versus Alexa-calmodulin fluorescence, reported in arbitrary fluorescence units; error bars indicate standard deviation with n = 4. Data were fit with equation (3) (dashed lines) and equation (4) (solid lines).

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