Figure 1

Myo1c constructs and IQ-domain sequences A, domain structure of Myo1c, indicating amino acids encompassed by full-length Myo1c and T701-Myo1c constructs. B, SDS-PAGE of 15 pmol each of purified full-length Myo1c and T701-Myo1c constructs co-expressed with calmodulin, and calmodulin alone (CaM; 45 pmol) on a 18% acrylamide gel. Molecular mass markers indicated on left. C, aligned bullfrog Myo1c IQ domains. The IQ motif positions are in bold and the repeat present in IQ3 and IQ4 is underlined. Numbers indicate amino-acid residue positions.