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Figure 6 | BMC Biochemistry

Figure 6

From: Inhibition of the MEK1/ERK pathway reduces arachidonic acid release independently of cPLA2 phosphorylation and translocation

Figure 6

Translocation of ECFP-cPLA 2 S727A following IONO stimulation Cells expressing ECFP-cPLA2S727A and EYFP-cPLA2 were stimulated with IONO. Representative frames from time-lapse images show the distribution of EYFP-cPLA2 and ECFP-cPLA2S727A fluorescence before and 13 s after addition of 10 μM IONO (panels A and B). (C) Fluorescent protein (FP) fluorescence from the area of the Golgi (inset) was analyzed with respect to time before and after IONO addition (arrow). (D) Overlay of the 77 s images from panels A and B. Results are representative of 4 independent experiments and analysis of several cells per experiment.

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BMC Biochemistry

ISSN: 1471-2091

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