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Figure 5 | BMC Biochemistry

Figure 5

From: Inhibition of the MEK1/ERK pathway reduces arachidonic acid release independently of cPLA2 phosphorylation and translocation

Figure 5

Translocation of ECFP-cPLA 2 S505A following ATP and IONO stimulation Cells expressing ECFP-cPLA2S505A and EYFP-cPLA2 were stimulated with ATP and IONO. ECFP and EYFP fluorescence images were taken at 3 s intervals. Representative frames from time-lapse images show the distribution of EYFP-cPLA2 and ECFP-cPLA2S505A fluorescence before and 30 s after addition of 100 μM ATP, followed by addition of 10 μM IONO at 1 min (panels A and B). (C) Fluorescent protein (FP) fluorescence from the area of the Golgi (inset) was analyzed with respect to time before and after ATP and IONO addition (arrows). (D) Overlay of the 75 s images from panels A and B. Results are representative of 5 independent experiments and analysis of several cells per experiment.

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