Figure 3
Effect of U0126 on ATP- and IONO-induced [Ca2+] i change MDCK cells were loaded with the fluorescent Ca2+ indicator Fura2, incubated with 10 μM U0126 or vehicle for 15 min, and stimulated with 100 μM ATP or 10 μM IONO. Graphs show changes in [Ca2+]i (expressed as the 340/380 ratio of Fura2 fluorescence) with respect to time from 8 individual cells (thin traces) and from the average of 16 cells (thick trace) treated with (A) U0126 and ATP, (B) vehicle and ATP, (C) U0126 and IONO, and (D) vehicle and IONO. Results are representative of 4 independent experiments in each condition and analysis of several cells per experiment.