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Table 1 Summary of the purification protocol of the protease produced by Trichoderma harzianum 1051.

From: Characterization of a protease produced by a Trichoderma harzianum isolate which controls cocoa plant witches' broom disease

Purification Step

Total activity (U)

Total protein (mg)

Specific activity (U/mg)

Purification (fold)

Yield(%)

*Freeze-dryed culture medium

274

5.0

55

-

100

** crude enzyme

220

7.5

29.3

1

80

***phenyl-Sepharose eluate

75

0.63

119

4.06

27

  1. *Freeze-dried culture medium ressuspended with 20 ml of 50 mM phosphate buffer, pH 5.0; **pellet resulting from precipitation of the freeze-dried material, with 60% (NH4)2 SO4 and resuspended with 3 ml of the phosphate buffer; *** Protease sample (Fig. 1, fractions 30–37) resulting from chromatography of the crude enzyme on a phenyl-Sepharose column.