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Figure 3 | BMC Biochemistry

Figure 3

From: MPN+, a putative catalytic motif found in a subset of MPN domain proteins from eukaryotes and prokaryotes, is critical for Rpn11 function

Figure 3

Accumulation of multiubiquitinated proteins in MPN motif mutants. Total cell extracts from WT yeast and from strains containing the MPN+ motif substitutions were separated on an 8% SDS gel and blotted with anti-ubiquitin antibodies. Cells were rapidly lysed in presence of 12% TCA in order to inhibit post lysis enzymatic activity. Accumulation of high MW polyubiquitinated proteins is detected in the Ser119 and Asp122 mutants, but not in WT or in the Cys116 mutants. His111 mutants accumulate polyubiquitinated proteins as well (not shown). A protein band migrating at around 20 kDa that is detected with the anti Ub antibody is used as an internal loading control.

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BMC Biochemistry

ISSN: 1471-2091

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