Figure 6

Specificity of triplex formation To determine the specificity of triplex formation, different pairs of non-matching TFOs and DNAs were tested for their ability to form a triplex. A) DS1 (0.5 μM) was annealed with 2.5 μM TFO1 or TFO2. B) DS2 (0.5 μM) was annealed with 2.5 μM TFO1 or TFO2. C) DS1 (0.5 μM) was annealed with 2.5 μM TFO1 or TFO3. Triplex formation between non-matching TFOs and DNAs was not detectable indicating a very high sequence specificity of this process.