Figure 5

Kinetics of association of TFO2 to DS2 A) Example of the association kinetics. DS2 (0.5 μM) and TFO2 (2.5 μM) were mixed in binding buffer and incubated at 37°C in the cuvette. The fluorescence emission spectra were recorded at various time points. B) The TAMRA fluorescence of the spectra shown in A was determined and the data fitted to an equation describing the kinetics of a bimolecular binding reaction.