Figure 4From: Specificity of DNA triple helix formation analyzed by a FRET assayEquilibrium binding constant of TFO2 to DS2 A) To measure the binding affinity of TFO2 to DS2, DS2 (0.5 μM) was incubated with different amounts of TFO2 (10 nM to 10 μM) in binding buffer for 10 hours and the fluorescence spectra recorded. B) The TAMRA fluorescence was determined and fitted to an equation describing a bimolecular binding equilibrium.Back to article page