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Figure 3 | BMC Biochemistry

Figure 3

From: Specificity of DNA triple helix formation analyzed by a FRET assay

Figure 3

Dependence of triplex formation on divalent cations To analyze the dependence of triplex formation on divalent cations, DS1 (0.5 μM) was annealed to TFO (10 μM) in binding buffer (20 mM HEPES pH 7.5, 50 mM Na-acetate, 10 mM MgCl2) and in buffer not containing Mg2+-ions (20 mM HEPES pH 7.5, 50 mM Na-acetate). In the Mg2+ free buffer, no triplex formation is detectable.

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