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Figure 2 | BMC Biochemistry

Figure 2

From: Specificity of DNA triple helix formation analyzed by a FRET assay

Figure 2

Triple helix formation analyzed by the FRET assay A) Fluorescence emission spectra were recorded for DS1 (0.5 μM), TFO1 (2.5 μM) and DS1 annealed to TFO1 in binding buffer. B) Same experiments with DS2 and TFO2. In both panels, a decrease in FAM fluorescence at 520 nm and an increase in TAMRA fluorescence at 580 nm is observed that is indicative of FRET.

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