Figure 8

cAMP up-regulates α₁ and β₁ sGC mRNAs in bovine chromaffin cells. Cells were incubated during 24 hours with either: vehicle (control), 100 μmol/L Sp-cAMPS-AM, 100 μmol/L Sp-cAMPS-AM plus 1 μmol/L H-89 (this compound was added 30 min before the addition of cAMP analogue), 1 μmol/L H-89, or 30 μmol/L Sp-PET-8Br-cGMPS. After treatment, total RNA was isolated and used for quantitative RT-PCR using specific primers designed for α₁ (A) and β₁ (B) subunits. Results are expressed as mean ± SE of two experiments performed in triplicate. Significant differences from control are indicated by ^*p < 0.05, ^**p < 0.01. ⁺⁺p < 0.01 indicates significant differences between cells treated with Sp-cAMPS-AM and cells treated with Sp-cAMPS-AM plus H-89.