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Figure 3 | BMC Biochemistry

Figure 3

From: Prolonged exposure of chromaffin cells to nitric oxide down-regulates the activity of soluble guanylyl cyclase and corresponding mRNA and protein levels

Figure 3

Effect of intracellular levels of reduced glutathione (GSH) on basal (A) or NO-stimulated (B) cGMP accumulation in control or DETA/NO pretreated cells. Cells were incubated with the membrane permeable reduced glutathione (GSH-ME) (2 mmol/L) or with 0.5 mmol/L buthionine-(S,R)-sulfoximine (BSO) for 48 hours in the presence or absence of 50 μmol/L DETA/NO. The cells were then incubated for 30 min with 0.5 mmol/L IBMX and basal cGMP levels (A), or DEA/NO-stimulated cGMP levels (B) determined by radioimmunoassay. Significant differences from values obtained in DETA/NO-untreated cells (empty column) are marked: ***p < 0.001; **p < 0.01; *p < 0.05 (Paired student's t test). Significant differences from values obtained in control cells are marked: +p < 0.05 (Paired student's t test).

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