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Figure 3 | BMC Biochemistry

Figure 3

From: Delineation of RAID1, the RACK1 interaction domain located within the unique N-terminal region of the cAMP-specific phosphodiesterase, PDE4D5

Figure 3

Identification of residues involved in the interaction between PDE4D5 and RACK1 using a 2-hybrid screen. Shows a typical experiment of one done at least 3 times, where mutations of individual or pairs of residues in the indicated N-terminal region of full length PDE4D5 were expressed as LexA fusions of the non-mutated PDE4D5 (1–746), pGADN-RACK1 and LexA alone ('vector'). The interaction between the oncoproteins RASv12 and RAF served as a positive control

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BMC Biochemistry

ISSN: 1471-2091

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