Figure 6

Copper-Phenanthroline footprint analysis of ARS DNA fragment bound to p35. ARS DNA fragment was 5' end labelled at the T rich strand and incubated with p35 (300 mM salt eluate) and finally subjected to gel retardation assay. The gel was then treated with copper-phenanthroline mixture and DNA from bound (B) and unbound (U) fractions were extracted from gel, denatured and loaded in a sequencing gel. A+G ladder was also loaded to figure out the position of footprint. The relative positions of A, B1 and B2 boxes were indicated. The protected regions are shown by solid lines and asteric (^*). The hypersensitive site is denoted by arrowhead.