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Figure 4 | BMC Biochemistry

Figure 4

From: A ~35 kDa polypeptide from insect cells binds to yeast ACS like elements in the presence of ATP

Figure 4

Purification of p35 by gel filtration chromatography. (A) Gel retardation assay. 300 mM salt eluate (as shown in fig. 3B) was dialysed against Buffer H/0.15 and passed through Superose-12 Gel filtration column. Fractions 13–25 were tested for ARS 240bp DNA binding activity. Only fraction 15 showed a band shift compared to the free probe. Molecular mass markers are shown on the top. (B) Silver stain of gel filtration fractions. 0.25 ml of each gel filtration fractions (fraction 13–17) were precipitated using trichloro acetic acid (TCA). The samples were run in SDS-PAGE and proteins were visualised by silver stain. p35 was found only in the fraction 15.

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