Figure 1

Purification of GST-Cdc6 and its DNA binding activity. (A) SDS-PAGE of GST-Cdc6 purified by glutathione agarose beads purification technique from Sf9 insect cells infected with baculovirus expressing GST-Cdc6. The polypeptide was visualised by commassie stain. Molecular mass markers are indicated on the right. (B) Gel retardation assays were performed in the presence of reduced glutathione eluted GST or GST-Cdc6 fusion protein. S. cerevisiae ARS1 fragment (240 bp) was end labelled using γ-³²P ATP and incubated with GST or GST-Cdc6 as described in the materials and methods. Both GST and GST-Cdc6 showed strong band shifts.