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Table 1 Stability of wild type and mutated AChE (mean ± SEM) and an index of the increase in stability i.e. the ratio between the measurement made for the mutant and the wild type enzymes.

From: Improvement of Drosophila acetylcholinesterase stability by elimination of a free cysteine

Denaturation

Wild type

mutant

ratio

t50 at 45°C in min.

17.4 ± 2.1 (n = 10)

24.1 ± 4.3 (n = 5)

1.4

t50 in 4 M urea in min.

12.5 ± 1.2 (n = 7)

34.2 ± 2.0 (n = 5)

2.7

Recovery after freezing

0.24 ± 0.04 (n = 8)

0.49 ± 0.04 (n = 7)

2

t50 at room temperature in days

1.5 ± 0.2 (n = 5)

3.3 ± 0.5 (n = 4)

2.2

t50 in 20% acetonitrile in min.

0.94 ± 0.1 (n = 12)

1.4 ± 0.4 (n = 5)

1.5

t50 in presence of 0.1 mg/ml pronase

7.9 ± 0.2 (n = 9)

10.0 ± 0.6 (n = 9)

1.3

t50 in H2O2 in min.

4.6 ± 0.5 (n = 6)

4.0 ± 0.4 (n = 6)

0.9