From: Improvement of Drosophila acetylcholinesterase stability by elimination of a free cysteine
Denaturation | Wild type | mutant | ratio |
---|---|---|---|
t50 at 45°C in min. | 17.4 ± 2.1 (n = 10) | 24.1 ± 4.3 (n = 5) | 1.4 |
t50 in 4 M urea in min. | 12.5 ± 1.2 (n = 7) | 34.2 ± 2.0 (n = 5) | 2.7 |
Recovery after freezing | 0.24 ± 0.04 (n = 8) | 0.49 ± 0.04 (n = 7) | 2 |
t50 at room temperature in days | 1.5 ± 0.2 (n = 5) | 3.3 ± 0.5 (n = 4) | 2.2 |
t50 in 20% acetonitrile in min. | 0.94 ± 0.1 (n = 12) | 1.4 ± 0.4 (n = 5) | 1.5 |
t50 in presence of 0.1 mg/ml pronase | 7.9 ± 0.2 (n = 9) | 10.0 ± 0.6 (n = 9) | 1.3 |
t50 in H2O2 in min. | 4.6 ± 0.5 (n = 6) | 4.0 ± 0.4 (n = 6) | 0.9 |