Table 2 Nucleotide substrate utilisation by hAps1, hAps1-39N and hAps2
Nucleotide | Rate (nmol/min/μg) | Major products | ||
|---|---|---|---|---|
hAps1 | hAps1-39N | hAps2 | ||
Ap6A | 0.25 ± 0.04 | 0.19 ± 0.01 | 0.28 ± 0.04 | AMP + p5A |
Ap5A | 0.41 ± 0.05 | 0.37 ± 0.02 | 0.9 ± 0.1 | AMP + p4A |
Ap4A | 0.02 ± 0.01 | 0.02 ± 0.01 | 0.09 ± 0.03 | AMP + ATP |
Ap3A | 0 | 0 | 0 | |
Ap2A | 0 | 0 | 0 | |
Gp5G | 0.04 ± 0.02 | 0.02 ± 0.01 | 0 | |
Gp4G | 0.04 ± 0.02 | 0.04 ± 0.02 | 0.11 ± 0.04 | GMP, GDP, GTP |
Gp3G | 0 | 0 | 0 | |
Gp2G | 0 | 0 | 0 | |
Ap5G | 0 | 0 | 0 | |
Ap4G | 0 | 0 | 0 | |
p4A | 0.07 ± 0.03 | 0.10 ± 0.03 | 0.13 ± 0.04 | ATP |
ATP | 0 | 0.03 ± 0.02 | 0 | |
ADP-ribose | 0 | 0 | 0 | |
ADP-glucose | 0 | 0 | 0 | |
dATP | 0 | 0.07 ± 0.04 | 0 | |
p4G | 0.15 ± 0.05 | 0.21 ± 0.05 | 0.21 ± 0.03 | GTP |
GTP | 0.02 ± 0.01 | 0.03 ± 0.01 | 0 | |
GDP-glucose | 0.01 ± 0 | 0 | 0 | |
GDP-mannose | 0 | 0 | 0 | |
dGTP | 0.05 ± 0.03 | 0.05 ± 0.03 | 0 | |
dUTP | 0.04 ± 0.03 | 0.05 ± 0.02 | 0 | |
dTTP | 0.05 ± 0.04 | 0.05 ± 0.03 | 0 | |
CTP | 0.03 ± 0.02 | 0.03 ± 0.02 | 0 | |