Figure 7
From: Molecular cloning and tissue distribution of mammalian L-threonine 3-dehydrogenases
Expression of L-threonine dehydrogenase mRNA in tissues by semiquantitative PCR. (A) The expression level in each tissue cDNA was normalised to the expression level of the housekeeping gene β-actin. The ratio of L-threonine dehydrogenase mRNA to β-actin mRNA (Y axis, arbitrary units) from each tissue was standardised to that of liver, which was taken as 100. (B) Ethidium bromide stained agarose gel of PCR products after 40 cycles of amplification. Threonine dehydrogenase, TDH; 2-amino-3-ketobutyrate coenzyme A ligase, KBL. The tissues examined were: brain, Br; Heart, He; kidney, Ki; liver, Li; lung, Lu; skeletal muscle, SM; spleen, Sp; testis, Te; and no cDNA control, -c; 100 bp ladder, m.