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Table 3 Steady-State kinetic parameters of mutant HIV-1 RT carrying insertion in the β7–β8 loop of either or both the subunits

From: Insertion of a small peptide of six amino acids into the β7–β8 loop of the p51 subunit of HIV-1 reverse transcriptase perturbs the heterodimer and affects its activities

 

Poly (rA).(dT)18

U-5 PBS 49-mer DNA/17 mer

Enzyme

Km (dTTP) (μM)

kcat (S-1)102

kcat/Km (S-1M-1) × 104

Km (dNTP) (μM)

kcat (S-1)103

kcat/Km (S-1M-1) × 104

p66 WT /p66 WT

5.01

135.7

27.08

2.10

15.8

0.754

p66 WT /p51 WT

5.48

103.7

18.93

3.91

25.0

0.641

p66 INS /p66 INS

46.39

0.198

0.0043

6.05

0.400

0.0063

p66 INS /p51 WT

2.57

43.7

1.702

5.47

15.00

0.270

p66 WT /p51 INS

4.86

89.8

18.490

2.35

15.40

0.655

p66 INS /p51 INS

3.35

0.15

0.0448

5.71

0.300

0.0046

  1. Kinetic parameters of the wild type HIV-1RT and its mutant derivatives were determined on homopolymeric RNA (poly rA) and heteropolymeric DNA (U5-PBS 49-mer) templates with (dT)18 and 17 mer DNA as primers, respectively. The values obtained are the average of three independent experiments.