From: Identification of critical residues in loop E in the 5-HT3ASR binding site
Receptor | Kd (nM) [3H]granisetron | Bmax (pmol/mg protein) | n |
---|---|---|---|
Wildtype | 0.98 ± 0.12 | 3.2 ± .13 | 1 ± 0.12 |
Y140A | 2.7 ± 0.19 | 0.44 ± .08 | 1.5 ± 0.14 |
V141A | 2.5 ± 0.35 | 0.32 ± .04 | 1.3 ± 0.22 |
Y142A | 4.5 ± 0.5 | 0.97 ± .14 | 1.6 ± 0.27 |
V143A | 1.9 ± 0.28 | 1.1 ± .04 | 0.97 ± 0.13 |
H145A | 1.3 ± 0.13 | 0.81 ± .07 | 1.38 ± 0.17 |
R146A | 2.4 ± 0.34 | 1.0 ± .16 | 1.26 ± 0 .21 |
G147A | - | - | - |
E148A | 5.2 ± 1.2 | 5.5 ± 1.2 | 0.93 ± 0.19 |
V149A | - | - | - |
Q150A | 6.3 ± 0.45 | 0.56 ± .09 | 1.19 ± 0 .1 |
N151A | 6.9 ± 0.48 | 4.9 ± 1.1 | 1.66 ± 0.16 |
Y152A | 7.8 ± 1.1 | 0.59 ± .10 | 1.4 ± 0.24 |
K153A | 7.4 ± 0.61 | 0.30 ± .08 | 1.51 ± 0.17 |