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Figure 3 | BMC Biochemistry

Figure 3

From: Identification of critical residues in loop E in the 5-HT3ASR binding site

Figure 3

Saturation binding isotherms for binding of [3H]granisetron to wildtype, Y140A, Y142A and Y152A receptors. The data shown represent the specific binding of [3H]granisetron as determined from at least four identical experiments. Specific binding was determined as the fraction of total binding not inhibited by a saturating concentration of m CPBG or MDL-72222. Data were combined and fitted using non-linear curve fitting. Only small shifts in binding affinity to [3H]granisetron were observed on all three mutants. Kd values, Bmax and Hill coefficients are shown in Table 2.

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