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Table 3 PI(4,5)-P2-binding of mutants assayed by gel filtration

From: Mutational analysis of human profilin I reveals a second PI(4,5)-P2 binding site neighbouring the poly(L-proline) binding site

 

[PI(4,5)-P2]50%

Human profilin I

29

R74A

39

R74E

64

R88A

62

R135D

45

R136D

159

S56E/R74A

44

S56E/R74E

50

S56E/R88E

82

R74E/R88E

85

R135A/R136A

52

R88A/R136D

260

R88E/R136D

679

W3A

30

rat profilin IIa

155

W3A

27

  1. PI(4,5)-P2-binding of mutants assayed by gel filtration (see Figure 5). Listed are the PI(4,5)-P2 concentrations where 50% of profilin is bound to the PI(4,5)-P2 micelles. In case of R88E/R136D, C50% was not reached and the listed value is an extrapolated value
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BMC Biochemistry

ISSN: 1471-2091

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