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Table 3 PI(4,5)-P2-binding of mutants assayed by gel filtration

From: Mutational analysis of human profilin I reveals a second PI(4,5)-P2 binding site neighbouring the poly(L-proline) binding site

  [PI(4,5)-P2]50%
Human profilin I 29
R74A 39
R74E 64
R88A 62
R135D 45
R136D 159
S56E/R74A 44
S56E/R74E 50
S56E/R88E 82
R74E/R88E 85
R135A/R136A 52
R88A/R136D 260
R88E/R136D 679
W3A 30
rat profilin IIa 155
W3A 27
  1. PI(4,5)-P2-binding of mutants assayed by gel filtration (see Figure 5). Listed are the PI(4,5)-P2 concentrations where 50% of profilin is bound to the PI(4,5)-P2 micelles. In case of R88E/R136D, C50% was not reached and the listed value is an extrapolated value