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Table 1 Interaction of wild type and mutant profilins with a proline-rich peptide

From: Mutational analysis of human profilin I reveals a second PI(4,5)-P2 binding site neighbouring the poly(L-proline) binding site

 

R.U. (200 μM)

R.U. (140 μM)

R.U. (100 μM)

Human profilin I

433

318

241

R74A

N.T.

240

180

R74E

280

194

146

R88A

280

212

163

S56E

258

211

167

R135D

N.T.

80

55

R136D

168

131

93

S56E/R74E

N.T.

N.T.

N.T.

S56E/R74A

224

177

137

S56E/R88E

167

141

105

R74E/R88E

N.T.

157

80

R135A/R136A

100

65

43

R88A/R136D

132

121

86

R88E/R136D

152

134

94

W3A

N.T.

N.T.

N.T.

rat profilin IIa

2041

1935

1890

W3A

N.T.

43

24

  1. (GP5)3 peptide binding was determined using Biacore technology. The reasonance units (R.U.) for three different concentrations of profilin were measured. N.T. is not tested.