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Table 1 Interaction of wild type and mutant profilins with a proline-rich peptide

From: Mutational analysis of human profilin I reveals a second PI(4,5)-P2 binding site neighbouring the poly(L-proline) binding site

  R.U. (200 μM) R.U. (140 μM) R.U. (100 μM)
Human profilin I 433 318 241
R74A N.T. 240 180
R74E 280 194 146
R88A 280 212 163
S56E 258 211 167
R135D N.T. 80 55
R136D 168 131 93
S56E/R74E N.T. N.T. N.T.
S56E/R74A 224 177 137
S56E/R88E 167 141 105
R74E/R88E N.T. 157 80
R135A/R136A 100 65 43
R88A/R136D 132 121 86
R88E/R136D 152 134 94
W3A N.T. N.T. N.T.
rat profilin IIa 2041 1935 1890
W3A N.T. 43 24
  1. (GP5)3 peptide binding was determined using Biacore technology. The reasonance units (R.U.) for three different concentrations of profilin were measured. N.T. is not tested.