Figure 4

Parallel -5/2_|| region of VO²⁺-EPR spectra in the presence of different nucleotides and conditions. a. One equivalent of a 1 : 1 mole ratio of VO²⁺-AMPPCP was added to sCD39, and incubated on ice for 5 minutes. b. The sample from a was thawed and incubated at room temperature for 30 minutes before collecting data again. c. One equivalent of a 1 : 1 mole ratio of VO²⁺-ATP was added to sCD39, and frozen immediately in liquid nitrogen. d. The sample from c was thawed and incubated at room temperature for 30 minutes before collecting data again.