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Figure 3 | BMC Biochemistry

Figure 3

From: The Metal Coordination of sCD39 during ATP Hydrolysis

Figure 3

Parallel regions of VO2+-EPR spectrum of VO2+-AMPCP bound to sCD39. a. One mole equivalent of VO2+-AMPCP was added to 6.5 mg of sCD39 prepared as in Experimental Procedures. EPR conditions were as follows: field modulation frequency, 100 kHz; modulation amplitude, 0.97 G; receive gain, 2 × 104; sweep time 5.243 s; time constant 1.28 ms; micropower, 1.0 mW; microwave frequency 9.40677 GHz, 1600 scans; temperature; 125 K. b. Simulated spectrum was generated by the program QPOWA with the experimental conditions above and A|| = 521.78 MHz, g|| = 1.9370 for Species D1. c. Simulated spectrum was generated by the program QPOWA with the experimental conditions above and A|| = 490.01 MHz, g|| = 1.9435 for Species D2.

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