Figure 5
From: Regulation of prostaglandin synthesis and cell adhesion by a tryptophan catabolizing enzyme
Phenotype of IDO-antisense transfected P19 cells. (A-C) P19 cells growing as a monolayer. (A) IDO-sense (B) IDO-antisense transfected P19 clone D3 (C) IDO antisense clone E6, growing as a monolayer. Bar:1 mm. (D-E); P19 cells growing as aggregates. (D) Aggregates from IDO sense-transfected P19 cells, (E) aggregates from IDO antisense-transfected P19 clone D3 grown in suspension for 30 hours in the presence of 1% DMSO. (F) Clone E6 treated as for D3. Bar: 50 mm (G) A representative field at 10x magnification was selected and the areas of 50 individual aggregates was calculated for both sense and antisense clone E6. Results are shown as the percentage of the total aggregates analyzed whose areas are within a given 0,2 sq. inch interval. (H) Percentage of IDO-sense and-antisense transfected P19 cells migrating to lower chamber of 24 well tissue culture plates18 hours following seeding. S: sense control, E6, D3, C2: antisense clones.