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Figure 1 | BMC Biochemistry

Figure 1

From: Regulation of prostaglandin synthesis and cell adhesion by a tryptophan catabolizing enzyme

Figure 1

Characterization of RAW cell transfectants. (A) IDO construct used to transfect RAW cells showing X, Y and W/S boxes of MHC Class II promoter. (B) Relative copy number determination of IDO-transfected RAW cell clones. The rabbit β globin intron present in the construct was used as a probe for hybridization by slot blot. (C) RT-PCR of total RNA isolated from RAW transfectants following 15 cycles of RT-PCR, electrophoresis on 0.8% agarose, followed by Southern blotting and hybridization with an IDO specific probe. (D) Western blot of IDO-expressing RAW cell transfectants, using IDO-specific polyclonal antibody. (E) HPLC determination of tryptophan depletion from tissue culture medium by IDO-expressing RAW cells, 48 hours post-seeding into fresh medium. V; Vector-only control transfectant. Clones 6, 8, 11, 22; IDO-expressing RAW clones.

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