Figure 1
From: Osmotic stress-dependent serine phosphorylation of the histidine kinase homologue DokA
Identification of overexpressed DokA fragments. Cells transformed with the pDEX-RH-dokA constructs were lysed in Laemmli sample buffer and subjected to SDS-PAGE. Proteins were blotted onto a PVDF membrane and DokA fragments were detected by immunostaining with the pAb PHKR antibody. The 99 kd fragment PHKR (Panel A, lane 1) and the 19 kd fragment RR (Panel B, lane 1) are expressed at comparable levels whereas the 51 kd fragment HK (Panel A, lane 2) is more weakly expressed. Wild-type DokA in AX2 cell extracts can not be detected (Panel A, lane 3; panel B, lane 2). All constructs used in this work are represented in a schematic drawing (Panel C).