Figure 7
From: Reconstitution of licensed replication origins on Xenopus sperm nuclei using purified proteins
Nucleotide requirement for Cdc6 chromatin association in desalted extracts. Sperm nuclei were incubated in desalted whole egg extract plus or minus geminin in the presence of either ADP, ATP, ATP-γ-S at 2.5 mM, or no added nucleotide. a, The degree of functional licensing was assessed, expressed as % of the DNA template replicated. b, Chromatin was isolated and immunoblotted for the presence of Cdc6.