Figure 5From: Reconstitution of licensed replication origins on Xenopus sperm nuclei using purified proteinsRequirements for Cdt1 and geminin association with chromatin. a, b, Xenopus sperm nuclei were incubated with the indicated proteins in the presence of 2.5 mM ATP. Chromatin was then isolated and immunoblotted for the presence of bound Orc1, Cdt1 and geminin. The RLF-B/Cdt1 was a slightly more active fraction taken from the geminin bead eluate.Back to article page