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Figure 4 | BMC Biochemistry

Figure 4

From: Reconstitution of licensed replication origins on Xenopus sperm nuclei using purified proteins

Figure 4

Reconstitution of licensing using purified proteins. a, c, d, Licensing activity of different combinations of purified proteins incubated in the presence of 2.5 mM ATP. DNA synthesis is expressed as % of the DNA template replicated. b, Western blot of Mcm7 loaded onto sperm chromatin in whole Xenopus extract, the full reconstituted reaction and the reconstituted reaction lacking RLF-B/Cdt1. The reconstituted reactions contained 2.5 mM ATP.

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