Figure 3

SDS-PAGE (10%) of pooled and concentrated column-purified fractions. Molecular weights of marker bands (M) are at left. Lane 1, purified BtR175 from size-exclusion fractions 9–11 (Fig. 2B); Lane 2, anion-exchange column fractions 26–27 (Fig. 1) used for size-exclusion purification of BtR175; Lane 3, purified APN from size-exclusion fractions 15–16 (Fig. 2A); Lane 4, anion-exchange column fractions 33–36 (Fig. 1) used for size-exclusion purification of APN.