Figure 1

Characterization of mAb 6B12. A. HT1080 cells were surface labeled with ¹²⁵I, lysed in the indicated detergents (each at 1%), and then proteins were immunoprecipitated using mAb 6B12 (lanes a,b). Also, ¹²⁵I-labeled Molt4 cells were lysed in 1% Triton XI 00, and labeled proteins were precipitated using mAb 6B12 (lane c), 4F2 (lane d), or anti-mAb A2-2E10 (lane e). Proteins were resolved on 10% SDS-PAGE under reduced conditions. B. Chinese hamster ovary cells transfected with CD98 cDNA (solid line), with β1 integrin cDNA (close dotted line), or mock transfected (dotted line) were each stained with mAb 6B12. Following FITC-labeled goat anti-mouse second antibody staining, flow cytometry was carried out using a FACScan Instrument (Becton Dickinson, Mountainview, CA) as previously described [30].