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Table 1 Influence of plasma lipases on the 3H-free cholesterol (FC) and 14C-phospholipid (PL) transfer from lipid emulsions to the human plasma lipoproteins.

From: Plasma lipases and lipid transfer proteins increase phospholipid but not free cholesterol transfer from lipid emulsion to high density lipoproteins.

Fractions

Control

 

Post-heparin

Post-heparin +

THL

 
 

3H-FC

14C-PL

3H-FC

14C-PL

3H-FC

14C-PL

LDL

15

7

21

7

20

6

 

(2-35)

(3-49)

(15-26)

(2-13)

(17-34)

(1-12)

HDL

13

31a

18

50a,b

15

39a,c

 

(7-28)

(18-47)

(12-36)

(35-68)

(8-33)

(23-48)

  1. Percent transfer of 3H-FC and 14C-PL from lipid emulsion to LDL and HDL obtained from fasting human plasma control, post-heparin with and without addition of a lipase inhibitor, tetrahydrolipstatin (THL) incubated for 30 minutes at zero (ice) and at 37°C. Plasma lipoproteins were fractionated by FPLC. Percent transfer was calculated as the difference between values obtained at 37°C and 0°C. Results are expressed as median (range), n=12. Mann-Whitney test: a: p< 0.001 (HDL vs. LDL) ; b: p<0.001 (post-heparin vs. control) and c: p<0.01 (post-heparin vs. post-heparin+THL).