Figure 5
Effect of dsRNA on the ex-vivo growth of the Plasmodium falciparum within red blood cells. Parasite growth evaluated by the SYBER green assay using synchronized cultures. Figure A. shows ds RNA preparation of green fluorescent protein (GFP) (lane 1) and PfUDN (lane 2) lane M is the marker. B. shows the growth of culture 50 hour post dsRNA treatment (i.e. at 56 hour stage of parasite growth) with the dsRNA of GFP (lane 1 in A), and PfUDN (lane 2 in A). C. Giemsa-stained parasite infected RBC after dsRNA treatment. Control panel in (i) shows the culture after treatment with GFP-dsRNA while panel (ii) shows the culture after treatment with dsRNA of PfUDN at different time interval as labeled. The arrows in each panel show P. falciparum infected RBC at different developmental stages.