Figure 1
Sequence map of the cloning of Pv D 1 into the expression vector pET-32 EK/LIC. The coding sequence of the Pv D1 (in bold) is shown linked to the LIC site of the vector pET-32 EK/LIC (indicated by a vertical arrow). The T7 promoter and terminator (horizontal arrows), lac operator, thioredoxin (Trx), six consecutive histidines (His) and (S) cleavable tags, thrombin and enterokinase protease sites, and His tag and terminator codon (-) are shown on the sequence map. The relevant amino acid sequences are shown below the nucleotide sequence. After expression and purification of the recombinant protein in this vector, all of the tags can be separated from the recombinant Pv D1 by a combined treatment with enterokinase as well as another chromatographic step.